Unexpected sex-specific post-reproductive lifespan in the free-living nematode Pristionchus exspectatus

Patterns of senescence (or aging) can vary among life history traits and between the sexes, providing an opportunity to study variation in the aging process within a single species. We previously found that females of the nematode Pristionchus exspectatus outlive males by a substantial margin under laboratory conditions. Here, we show that sex-specific reproductive senescence unfolds in the opposite direction in this species, resulting in a prolonged period of female-specific post-reproductive survival: females lost the ability to reproduce at approximately 4.7 weeks despite a median lifespan of about 12.3 weeks under lab conditions, whereas males lost the ability to reproduce at approximately 6.6 weeks, roughly in line with their median lifespan of around 7.6 weeks. Interestingly, somatic senescence (declining crawling speed) only explained reproductive senescence in males, whereas females lost the ability to reproduce regardless of condition. However, we found that housing females with males significantly increased their mortality rate, indicating that female-specific post-reproductive survival is unlikely to occur in the wild. We discuss our results in light of evolutionary theories of post-reproductive survival and previous studies of nematode behavioral ecology, arguing that premature reproductive senescence may stem from sex-specific condition-dependent survival during the reproductive period. Given the proven lab tractability of Prisitonchus nematodes, our findings provide a foundation for integrative research that combines evolutionary ecology and molecular genetics in the study of sex-specific senescence and post-reproductive survival.