Related Experiment Video
Updated: Mar 11, 2026
10:38
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Published on: September 27, 2012
23.3K
Unmasking ultradian rhythms in gene expression
Daan R van der Veen1, Menno P Gerkema2
1Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom; and d.vanderveen@surrey.ac.uk.
Summary
This study reveals intrinsic ultradian rhythms in gene expression, with over 900 genes showing ultradian patterns in vitro. These findings suggest underlying molecular mechanisms for these biological oscillations.
Area of Science:
- Chronobiology
- Molecular Biology
- Genetics
Background:
- Biological oscillations occur on various timescales, including ultradian (1-several hours) and circadian rhythms.
- Ultradian rhythms have been presumed to have external origins due to a lack of in vitro evidence and known molecular oscillators.
Purpose of the Study:
- To develop a novel method for distinguishing ultradian from circadian gene expression components.
- To investigate the presence and molecular basis of intrinsic ultradian rhythms in gene expression in vitro.
Main Methods:
- A new non-spectral analysis technique was employed to separate ultradian and circadian gene expression patterns.
- The method was applied to a gene expression dataset with high temporal resolution (ultradian sampling).
- Validation was performed using existing in vivo data from mouse hepatocytes.
Main Results:
- Over 900 genes exhibited ultradian expression patterns in vitro, challenging previous assumptions.
- Sixty genes demonstrated ultradian transcriptional rhythmicity in both in vivo and in vitro settings.
- Enrichment of specific DNA motifs in the promoters of these 60 genes suggests regulatory mechanisms.
Conclusions:
- The study provides strong evidence for intrinsically driven ultradian rhythms in gene expression.
- Potential molecular mechanisms and functions underlying these ultradian rhythms have been identified.
- This research opens new avenues for understanding biological timing beyond circadian control.
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