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Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
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Insights into the human mesenchymal stromal/stem cell identity through integrative transcriptomic profiling.
Beatriz Roson-Burgo1,2,3, Fermin Sanchez-Guijo2,3, Consuelo Del Cañizo2,3
1Bioinformatics and Functional Genomics Group, Cancer Research Center (IBMCC, CSIC/USAL) and IBSAL, Consejo Superior de Investigaciones Cientificas (CSIC), Salamanca, Spain.
BMC Genomics
|November 23, 2016
Summary
Researchers defined a core mesenchymal lineage signature of 489 genes to better characterize human Mesenchymal Stromal/Stem Cells (MSCs). This signature aids in distinguishing MSCs from other cell types, improving cell therapy applications.
Area of Science:
- Stem Cell Biology
- Genomics
- Biomolecular Characterization
Background:
- Human Mesenchymal Stromal/Stem Cells (MSCs) possess a poorly characterized phenotype, making them difficult to distinguish from similar cell populations.
- A comprehensive comparative analysis of MSCs' gene expression profiles within a relevant cellular context is lacking.
- There is a need for a well-defined MSC gene signature to understand their role in crucial cellular processes like immunomodulation and differentiation.
Purpose of the Study:
- To define a core mesenchymal lineage gene signature for human MSCs.
- To conduct a deep comparative analysis of MSC expression profiles across different tissue origins and commitment states.
- To identify novel CD marker patterns and cytokine-receptor profiles for MSC subtypes.
Main Methods:
- Integration of multiple public transcriptomic expression datasets.
- Generation of de-novo microarray and RNA-Seq datasets.
- Comparative analysis of MSCs from various tissue origins (adipose, placenta, bone marrow) and related non-mesenchymal cells.
Main Results:
- A core mesenchymal lineage signature comprising 489 genes was defined.
- Tissue-specific signatures were identified for adipose, chorionic placenta, and bone marrow MSCs, clarifying the MSC-fibroblast relationship.
- Novel CD marker patterns and cytokine-receptor profiles were revealed, with MCAM (CD146) identified as a key marker for bone marrow MSCs.
Conclusions:
- The study provides an improved biomolecular characterization of human MSCs.
- The released genome-wide expression signatures offer a valuable resource for future functional studies.
- These findings can drive the redesign of more effective cell therapy applications.
