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Related Experiment Video

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Harmonising Reference Intervals for Three Calculated Parameters used in Clinical Chemistry.

David Hughes1, Gus Koerbin2, Julia M Potter3

  • 1ACT Pathology, ACT, 2605, Australia.

The Clinical Biochemist. Reviews
|November 23, 2016
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Summary
This summary is machine-generated.

Harmonizing laboratory testing is crucial. This study established reference intervals for calculated serum osmolality, serum anion gap, and albumin-adjusted serum calcium, finding serum osmolality intervals potentially suitable for common use.

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Area of Science:

  • Clinical chemistry and laboratory medicine
  • Biomarker standardization
  • Reference interval establishment

Background:

  • Global efforts aim to harmonize laboratory testing, including calculated parameters derived from measurands.
  • Standardized reference intervals are essential for consistent clinical interpretation and inter-laboratory comparisons.
  • The Aussie Normals study provides a foundation for establishing harmonized reference intervals for key calculated analytes.

Approach:

  • Analyzed data from 1856 healthy volunteers in the Aussie Normals study.
  • Determined reference intervals for serum osmolality, serum anion gap, and albumin-adjusted serum calcium.
  • Evaluated suitability for harmonization using Royal College of Pathologists of Australasia/Australasian Association of Clinical Biochemists (RCPA/AACB) bias survey data.

Key Points:

  • Gaussian distributions observed for albumin-adjusted serum calcium, serum anion gap, and calculated serum osmolality.
  • Serum osmolality reference intervals derived from Smithline and Gardner formulae show potential for harmonization.
  • Albumin-adjusted serum calcium harmonization requires further investigation due to method biases (BCG vs. BCP).

Conclusions:

  • Calculated serum osmolality reference intervals using Smithline and Gardner formulae may be suitable for common use.
  • Further research is needed for albumin-adjusted serum calcium harmonization, addressing albumin concentration ranges and method biases.
  • Anion gap harmonization requires further study due to issues with Total CO2 measurement in bias surveys.