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Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
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Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein
Shogo Tsuji1, Shiroh Futaki1, Miki Imanishi1
1Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp.
Chemical Communications (Cambridge, England)
|November 23, 2016
Abstract:
A 5mC-selective TALE-repeat was created by screening a TALE repeat library containing randomized amino acids at repeat variable diresidues and their neighboring residues. The new repeat showed high 5mC discrimination ability. An artificial TALE containing the new repeat activated an endogenous gene in a genomic methylation status-dependent manner.
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