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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Synthetic Glycan Microarrays.

Felix Broecker1,2, Peter H Seeberger3,4

  • 1Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476, Potsdam, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2016
PubMed
Summary

Synthetic glycan microarrays enable the study of glycan-binding protein interactions, aiding in disease research and diagnostics. These custom microarrays allow for the precise examination of specific glycan-GBP binding, advancing biological and biomedical applications.

Keywords:
Automated glycan assembly (AGA)GlycanGlycan binding protein (GBP)Glycan–GBP interactionMicroarrayOligosaccharide

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Area of Science:

  • Carbohydrate Chemistry
  • Glycobiology
  • Biotechnology

Background:

  • Glycans are crucial molecules involved in numerous biological functions.
  • Glycan-binding protein (GBP) interactions are vital in human development, infection, and immunity.
  • Chemical synthesis, particularly automated glycan assembly, now provides access to complex glycans.

Purpose of the Study:

  • To describe methods for fabricating custom glycan microarrays.
  • To enable the examination of glycan-GBP binding specificities.
  • To facilitate research from basic science to biomedical applications.

Main Methods:

  • Synthetic glycans are prepared for conjugation.
  • Glycans are covalently attached to microarray surfaces via nucleophilic linkers.
  • Microarrays are incubated with GBPs, and binding is detected using fluorescent signals.

Main Results:

  • Custom glycan microarrays can be fabricated using described methods.
  • The methods allow for quantitative detection of glycan-GBP binding events.
  • These microarrays are adaptable for diverse research and diagnostic purposes.

Conclusions:

  • Synthetic glycan microarrays are powerful tools for studying glycan-GBP interactions.
  • Fabrication methods are versatile and applicable to various research needs.
  • This technology supports advancements in understanding biological functions and developing diagnostic tools.