Related Experiment Video

Updated: Mar 11, 2026

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

7.1K

Expression and significances of MiRNA Let-7 and HMGA2 in laryngeal carcinoma

F-C Song1, Y Yang, J-X Liu

  • 1Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medical Center, Tianjin, China. yangyangtjin@163.com.

European Review for Medical and Pharmacological Sciences
|November 23, 2016
PubMed
Abstract

Insights

Low levels of microRNA Let-7a and high levels of High-mobility group AT-hook 2 (HMGA2) protein correlate with advanced laryngeal cancer. This suggests HMGA2 is an oncogene promoted by Let-7a down-regulation, driving cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • High-mobility group protein A2 (HMGA2) is an oncogene implicated in laryngeal carcinoma.
  • MicroRNA Let-7a acts as a tumor suppressor, regulating HMGA2 expression.
  • The interplay between Let-7a and HMGA2 is crucial in laryngeal cancer development.

Purpose of the Study:

  • To investigate the molecular mechanisms of Let-7a and HMGA2 in laryngeal cancer oncogenesis.
  • To determine the correlation between Let-7a and HMGA2 expression in laryngeal tissues.
  • To assess the role of Let-7a and HMGA2 in tumor progression and metastasis.

Main Methods:

  • Quantitative RT-PCR was used to measure Let-7a miRNA and HMGA2 mRNA expression.
  • Western blot analysis detected HMGA2 protein levels.
  • Spearman correlation analysis assessed the relationship between Let-7a and HMGA2.

Main Results:

  • A significant negative correlation was observed between Let-7a expression and HMGA2 expression (p < 0.05).
  • Expression levels differed significantly across clinical stages, differentiation grades, and lymph node metastasis.
  • Down-regulation of Let-7a and up-regulation of HMGA2 were associated with increased invasion and metastasis.

Conclusions:

  • MicroRNA Let-7a acts as a tumor suppressor in laryngeal cancer by inhibiting HMGA2.
  • The down-regulation of Let-7a and subsequent up-regulation of HMGA2 promote laryngeal cancer invasion and metastasis.
  • Targeting the Let-7a/HMGA2 pathway may offer therapeutic strategies for laryngeal carcinoma.

More Related Videos

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K
miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.3K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.1K