Use of chicken embryonated eggs for evaluating the virulence of Toxoplasma gondii

The objective of the present study was to examine the virulence of Toxoplasma gondii RH strain tachyzoites in embryonated eggs after continuous passage in Vero cell line. The first T. gondii tachyzoites was considered low passage (no passage) and then passaged for 80 times on Vero as high passage. Groups of 9-day-old embryonated eggs with ten eggs in each group were inoculated with 10²,10³ and 10⁴ of low or high-passage T. gondii tachyzoites, and any mortality was recorded. Suitable samples from different tissues (liver, heart, and brain) of the dead embryos were collected for histopathological study. In this study, the mortality in group 10³ and 10⁴ was observed, but there was no significant differences in mortality rate in T. gondii low passage and high passage. This finding exactly correspond to previous studies that were performed in mice as animal model for T. gondii RH strain. Thus on base of this study we could introduce the embryonated eggs as an appropriate animal model to evaluate the virulence of T. gondii tachyzoites.