Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

947
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
947
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.5K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.5K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

16.1K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
16.1K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.5K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.5K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.8K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for the Y(2175) in the Photoproduction Cross Section Measurement of γp→ϕπ^{+}π^{-}p at GlueX.

Physical review letters·2026
Same author

Neutron-Multiplicity Measurement in Muon Capture on Oxygen Nuclei in the Gadolinium-Loaded Super-Kamiokande Detector.

Physical review letters·2026
Same author

Results from the T2K Experiment on Neutrino Mixing Including a New Far Detector μ-like Sample.

Physical review letters·2026
Same author

Imlunestrant with or without abemaciclib in advanced breast cancer: updated efficacy results from the phase III EMBER-3 trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

First Differential Measurement of the Single π^{+} Production Cross Section in Neutrino Neutral-Current Scattering.

Physical review letters·2025
Same author

First Measurement of the Electron-Neutrino Charged-Current Pion Production Cross Section on Carbon with the T2K Near Detector.

Physical review letters·2025

Related Experiment Video

Updated: Mar 11, 2026

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

6.8K

Advanced three-dimensional electron microscopy techniques in the quest for better structural and functional

D Schryvers1, S Cao1, W Tirry1

  • 1EMAT, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.

Science and Technology of Advanced Materials
|November 24, 2016
PubMed
Summary

This study uses advanced electron tomography to reveal the 3D structure of metallic materials, focusing on shape memory alloys and nanostructures. Key findings detail precipitate morphology and distribution, offering new quantification methods for materials science.

Keywords:
62.20.fg62.25.-g68.37.-dNi–Tiatomic resolutionelectron tomographynanomechanicsprecipitationslice-and-view

More Related Videos

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.9K
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.8K

Related Experiment Videos

Last Updated: Mar 11, 2026

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

6.8K
Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.9K
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.8K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Metallurgy

Background:

  • Electron tomography is crucial for understanding complex 3D material structures.
  • Shape memory alloys and nanostructured metals exhibit unique properties influenced by their microstructure.
  • Characterizing precipitate morphology and distribution is key to predicting material behavior.

Approach:

  • Utilized FIB/SEM slice-and-view for 3D data acquisition in Ni-Ti alloys.
  • Applied various quantification techniques: principal ellipsoid, Zingg classification, particle distribution, distance transform, and water penetration.
  • Employed electron tomography to analyze defect interactions in Al nanobeams and Ag nanoparticles.

Key Points:

  • Detailed 3D morphology and distribution of Ni4Ti3 precipitates in binary Ni-Ti.
  • Novel water penetration method to quantify matrix transformation between precipitates.
  • Investigated precipitation in single crystals (layered/autocatalytic) and polycrystals (multistage transformation).
  • Analyzed focused ion beam-induced defects and dislocation structures in Al.
  • Demonstrated atomic resolution tomography on Ag nanoparticles in Al matrix.

Conclusions:

  • Electron tomography provides critical insights into the 3D microstructure of advanced metallic materials.
  • Advanced quantification techniques enhance understanding of precipitate behavior and matrix interactions.
  • This work advances the characterization of shape memory alloys and nanostructured systems.