Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suppressive Immune Response of Poly(sarcosine) Conjugated Proteins: PSARylation.

Bioconjugate chemistry·2026
Same author

Hydrosilylation of ω-hydroxyalkenes catalysed with 2-methacryloyloxyethyl-phosphorylcholine-protected ruthenium nanoparticles.

Chemical communications (Cambridge, England)·2026
Same author

Linear Force Scaling in Kinesin-Driven Microtubule Swarms Revealed by Electromagnetic Tweezers.

ACS nano·2026
Same author

Highly Sensitive Fluorometric Acetone Biosensor Using Hemi-Ellipsoidal Mirror Optics for Efficient Light Collection.

ACS sensors·2026
Same author

Poly(2-Methacryloxyethyl Phosphorylcholine-Co-Butyl Methacrylate) Coating Enhances Biomimetic Mineralization of Bioglass and Promotes Cellular Proliferation.

Tissue engineering and regenerative medicine·2026
Same author

Omega Nucleic Acids (ΩNA), Ultimate Nucleic Acids for Future Technology.

Molecules (Basel, Switzerland)·2026

Related Experiment Video

Updated: Mar 11, 2026

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

2.0K

Crosslinked duplex DNA nanogels that target specified proteins.

Yasuhiko Iwasaki1, Jun-Ichi Kondo1, Akinori Kuzuya1

  • 1Department of Chemistry and Materials Engineering; Faculty of Chemistry, Materials and Bioengineering, Kansai University , Suita-shi, Osaka , Japan.

Science and Technology of Advanced Materials
|November 24, 2016
PubMed
Summary

Researchers developed novel nanogels that specifically detect the cancer biomarker thrombin. These aptamer-crosslinked nanogels offer a promising platform for advanced disease diagnostics and targeted therapies.

Keywords:
101 Self-assembly/Self-organized materials208 Sensors and actuators211 Scaffold/Tissue engineering/Drug delivery30 Bio-inspired and biomedical materialsBiomarkernanogeloligonucleotide aptamerphospholipid polymer

More Related Videos

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.2K

Related Experiment Videos

Last Updated: Mar 11, 2026

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

2.0K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.2K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Specific detection of protein biomarkers is crucial for disease diagnosis and treatment.
  • Polymeric nanogels offer potential as advanced diagnostic and therapeutic tools.

Purpose of the Study:

  • To fabricate and characterize novel polymeric nanogels for the specific detection of the cancer biomarker thrombin.
  • To demonstrate the utility of aptamer-crosslinked nanogels as a versatile platform for disease diagnosis.

Main Methods:

  • Synthesis of 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymers with thrombin-binding aptamers via radical polymerization.
  • Formation of nanogels through complementary oligonucleotide strand association in aqueous media.
  • Characterization of nanogel formation, size, and monodispersity using dynamic light scattering and transmittance microscopy.
  • Incorporation of ethidium bromide (EtBr) as a functional intercalator for fluorescence-based detection.

Main Results:

  • Spontaneous formation of spherical, monodispersed nanogels (average size 124 ± 2 nm, PDI 0.21) was confirmed.
  • EtBr-incorporating nanogels showed decreased fluorescence intensity in the presence of thrombin due to structural transformation.
  • No significant fluorescence change was observed when nanogels were incubated with albumin, indicating specificity for thrombin.

Conclusions:

  • Aptamer-crosslinked nanogels provide a sensitive and specific method for detecting thrombin.
  • This nanogel platform is adaptable for detecting various biomarkers by designing specific oligonucleotide aptamers.
  • These nanogels represent a promising nanomaterial for future applications in disease diagnosis and therapy.