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Mass spectrometry based proteomics profiling of human monocytes
Yong Zeng1,2, Fei-Yan Deng2,3, Wei Zhu2,4
1College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Protein & Cell
|November 24, 2016
Summary
Researchers created a comprehensive human monocyte proteome knowledge base by integrating in vivo and in vitro data. This resource catalogs 2237 genes, aiding future studies on monocyte biology and diseases.
Area of Science:
- Immunology
- Proteomics
- Genomics
Background:
- Human monocytes are crucial in various diseases.
- Understanding monocyte biology requires comprehensive data integration.
Purpose of the Study:
- To develop the first comprehensive proteome knowledge base for human monocytes.
- To integrate in vivo and in vitro datasets for a unified view of monocyte gene expression.
Main Methods:
- Integrated top 2000 expressed genes from in vitro and 779 genes from in vivo datasets.
- Cataloged 2237 unique monocyte-expressed genes.
- Performed Gene Ontology (GO) analysis for functional annotation.
Main Results:
- Generated a core gene list of 541 unique genes from overlapping in vivo and in vitro data.
- Identified key genes (e.g., SAMHD1, G6PD) linked to immune response, blood biology, bone remodeling, and cancer.
- Performed gene-disease, pathway, and network analyses on the core gene list.
Conclusions:
- The developed knowledge base serves as a unique reference map for monocyte research.
- Facilitates in-depth studies on monocyte biology and associated human diseases.
- Provides biologically meaningful information through integrated data analysis.
