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Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
Amanda Herring1, Angelica Messana1, Anne M Bara1
1Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Stem Cell Research
|November 24, 2016
Abstract:
Here, we generated a biallelic mutation in the TLE1 (Transducin Like Enhancer of Split 1) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The homozygous knockout cell line, TLE1-464-G04, displays loss of TLE1 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
