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Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
Anne M Bara1, Angelica Messana1, Amanda Herring1
1Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Stem Cell Research
|November 24, 2016
Abstract:
Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
