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An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic

Enrique Sosa1, Rachel Kim1, Ernesto J Rojas1

  • 1Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Stem Cell Research
|November 24, 2016
PubMed

Insights

A new rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, was successfully generated from embryonic fibroblasts. This valuable primate stem cell resource aids research into developmental origins of disease and primate embryogenesis.

Area of Science:

  • Stem cell biology
  • Primate developmental biology

Background:

  • Induced pluripotent stem cells (iPSCs) offer a powerful model for studying human development and disease.
  • Non-human primate models are crucial for understanding primate-specific biology and disease mechanisms.

Purpose of the Study:

  • To generate and characterize a novel induced pluripotent stem cell line from the rhesus macaque.
  • To establish a valuable resource for studying primate embryonic development and disease.

Main Methods:

  • Rhesus embryonic fibroblasts (REFs) were isolated from embryonic day 47 rhesus macaque skin.
  • REFs were reprogrammed to generate induced pluripotent stem cells (riPSCs).
  • Karyotyping, stem cell marker analysis (OCT4, NANOG, TRA-1-81, SSEA4), and teratoma formation assays were performed.

Main Results:

  • A stable riPSC line, designated riPSC89, was successfully generated.
  • riPSC89 cells exhibited a normal male karyotype (42, XY).
  • The riPSC89 line expressed key markers of self-renewal and pluripotency and formed teratomas in vivo.

Conclusions:

  • The riPSC89 line represents a novel and well-characterized non-human primate pluripotent stem cell resource.
  • This riPSC line can be utilized to investigate the developmental origins of diseases.
  • riPSC89 serves as a fundamental model for understanding primate lineage specification.

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