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Affinity and Avidity01:41

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Improvement of Aptamer Affinity by Dimerization.

Hijiri Hasegawa1, Ken-Ichi Taira1, Koji Sode2

  • 1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.

Sensors (Basel, Switzerland)
|November 24, 2016
PubMed
Summary
This summary is machine-generated.

Designing aptamer dimers significantly enhances binding affinity for targets like thrombin and vascular endothelial growth factor (VEGF). This approach improves aptamer efficacy, offering a promising strategy for therapeutic development.

Keywords:
Aptameraviditydimerization

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers are single-stranded nucleic acids that bind targets with high specificity.
  • Antibody avidity inspires aptamer dimerization to enhance target binding.
  • Improving aptamer affinity is crucial for therapeutic applications.

Purpose of the Study:

  • To design and evaluate aptamer dimers for enhanced affinity and inhibitory activity.
  • To investigate the effect of dimerization on aptamers targeting thrombin and vascular endothelial growth factor (VEGF).

Main Methods:

  • Conjugation of two distinct thrombin-binding aptamers (15-mer and 29-mer) to form a dimer.
  • Conjugation of two identical anti-VEGF aptamers to create a dimer.
  • Affinity measurement using Kd values and assessment of inhibitory activity.

Main Results:

  • The thrombin aptamer dimer exhibited a Kd value 10 times lower than its monomeric components.
  • The thrombin aptamer dimer demonstrated superior inhibitory activity compared to the monomer.
  • The VEGF aptamer dimer showed significantly reduced Kd compared to the monomer.

Conclusions:

  • Aptamer dimerization effectively enhances binding affinity for protein targets.
  • Dimerized aptamers offer improved inhibitory potential compared to monomers.
  • This strategy holds promise for developing more potent aptamer-based therapeutics.