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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

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Related Experiment Video

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Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

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Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development.

Norimasa Iwanami¹, Katarzyna Sikora², Andreas S Richter³

¹Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Stuebeweg 51, 79108 Freiburg, Germany.

|November 24, 2016

Summary

Zebrafish genetic screens reveal new insights into T-cell development. Unexpectedly, pre-mRNA processing factors, like TNPO3, are crucial for T-cell differentiation in vertebrates.

Keywords:

T cell development genetic screen immunodeficiency mouse pre-mRNA splicing zebrafish

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Last Updated: Mar 11, 2026

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Area of Science:

Immunology
Developmental Biology
Genetics

Background:

Lymphocytes are key to vertebrate adaptive immunity.
Non-mammalian models are valuable for studying lymphocyte development.
Understanding T-cell differentiation requires identifying key genetic pathways.

Purpose of the Study:

To identify genes regulating early T-cell development using zebrafish.
To investigate the role of pre-mRNA processing in T-cell differentiation.

Main Methods:

Forward genetic screens in zebrafish to identify mutations affecting T-cell development.
Comparative analysis with known mammalian immune genes.
Functional studies of splice regulator TNPO3 in zebrafish and mice.

Main Results:

Identified major genetic pathways involved in T-cell development.
Discovered an enrichment of pre-mRNA processing genes among mutants.
TNPO3 deficiency impairs T-cell differentiation in both zebrafish and mice.

Conclusions:

Pre-mRNA processing is a critical, evolutionarily conserved aspect of T-cell development.
Splice regulators like TNPO3 play cell-type-specific roles in T-cell differentiation.