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CD11d β2 integrin expression on human NK, B, and γδ T cells
Gabrielle M Siegers1,2, Christy R Barreira1, Lynne-Marie Postovit2
1Molecular Medicine, Robarts Research Institute, The University of Western Ontario, London, Ontario, Canada.
Journal of Leukocyte Biology
|November 25, 2016
Summary
CD11d integrin is newly found on B cells, NK cells, and gamma-delta T cells (γδTc), but not alpha-beta T cells (αβTc). This discovery suggests new roles for CD11d in leukocyte function and disease.
Area of Science:
- Immunology
- Cell Biology
- Leukocyte Biology
Background:
- The CD11d integrin is known to be expressed on myeloid leukocytes.
- Its expression and function on lymphocyte subsets have not been previously investigated.
Purpose of the Study:
- To determine the expression of CD11d on various human lymphocyte subsets.
- To investigate the potential functional implications of CD11d expression on lymphocytes.
Main Methods:
- Utilized multicolor flow cytometry to analyze CD11d expression on peripheral blood mononuclear cells (PBMCs).
- Examined CD11d expression on B cells, NK cells, CD4+ and CD8+ αβ T cells (αβTc), and γδ T cells (γδTc).
- Assessed CD11d levels in freshly isolated cells and cells cultured over time with IL-2 supplementation.
Main Results:
- CD11d was highly expressed on NK cells, B cells, and γδTc, but notably absent on αβTc.
- Freshly isolated γδTc exhibited higher CD11d expression than αβTc, with significant inter- and intradonor variability.
- In primary culture, CD11d expression on γδTc increased over time and correlated with IL-2 supplementation.
- A higher proportion of Vδ1 γδTc expressed CD11d compared to Vδ2 γδTc, indicating subset-specific expression.
Conclusions:
- CD11d integrin is expressed on specific lymphocyte subsets, including NK cells, B cells, and γδTc.
- The differential expression of CD11d on γδTc subsets suggests distinct roles in immune responses.
- These findings broaden the understanding of CD11d's function in lymphocyte trafficking, tissue retention, and leukocyte biology in health and disease.
