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Zebrafish embryonic development is induced by carp sperm.

Thomas A Delomas1, Konrad Dabrowski2

  • 1School of Environment and Natural Resources, The Ohio State University, Columbus, OH, USA delomas.1@osu.edu.

Biology Letters
|November 25, 2016
PubMed
Summary

Common carp sperm can activate zebrafish eggs for haploid gynogenetic development, improving genetic screens. This method enhances purity of haploid zebrafish by preventing contamination from zebrafish sperm.

Keywords:
forward genetic screengynogenesishaploid screenspontaneous diploid

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Area of Science:

  • Developmental Biology
  • Aquaculture
  • Genetics

Background:

  • Haploid gynogenetic screens are crucial for efficient forward genetics and linkage analysis in fish.
  • UV-irradiated zebrafish sperm is the standard method for activating zebrafish oocytes in haploid screens.
  • Incomplete irradiation of zebrafish sperm can lead to contamination of haploid progenies.

Purpose of the Study:

  • To describe the use of UV-irradiated common carp sperm for activating zebrafish oocytes for haploid gynogenetic development.
  • To evaluate the embryonic development and viability of carp × zebrafish hybrids.
  • To establish a more robust method for generating pure haploid zebrafish.

Main Methods:

  • UV-irradiated common carp sperm was used to activate zebrafish oocytes.
  • Embryonic development and tissue formation (muscle, heart, nervous system) of the resulting hybrids were analyzed.
  • The viability of hybrids was assessed throughout development.

Main Results:

  • Carp × zebrafish hybrids exhibited characteristic embryonic development and functional tissue formation.
  • Hybrids were found to be inviable beyond the embryonic stages.
  • The use of heterologous sperm (carp) eliminated contamination from incompletely irradiated zebrafish sperm.
  • A unique zebrafish female showing spontaneous diploidization with carp sperm was identified.

Conclusions:

  • UV-irradiated common carp sperm is a viable alternative for inducing haploid gynogenesis in zebrafish.
  • This technique improves the purity of haploid zebrafish by preventing contamination.
  • The carp × zebrafish hybrid model offers insights into early development, despite inviability.
  • The identification of spontaneous diploidization highlights unique genetic maternal effects.