Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

812
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
812
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.5K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Printed MoS<sub>2</sub> memristive nanosheet networks for spiking neurons with multi-order complexity.

Nature nanotechnology·2026
Same author

IFIT3 Associates with m⁶A-Modified RNA to Restrict Hepatitis C Virus Infection.

bioRxiv : the preprint server for biology·2026
Same author

Restoration of Mucosa-Associated Invariant T-Cell Function in Healthcare-Associated Bacterial Infections Supports Recovery of Carbapenem Efficacy Against Resistant Bacteria Ex Vivo.

The Journal of infectious diseases·2026
Same author

Comparative genomic analyses of <i>Escherichia coli</i> ST405 strains from Pakistan.

mSystems·2026
Same author

Effects of a Text Message-Based Lifestyle Intervention on HbA1c and Health Behaviors in Older Adults with Prediabetes.

Nutrients·2026
Same author

A Study on Hollow Mesoporous Silica Nanoparticles with Long-Term Cycling.

Materials (Basel, Switzerland)·2025

Related Experiment Video

Updated: Mar 11, 2026

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.6K

Why cannot a β-lactamase gene be detected using an efficient molecular diagnostic method?

Kwang Seung Park1, Jung Hun Lee2, Moonhee Park3

  • 1Kwang Seung Park, National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Republic of Korea.

Pakistan Journal of Medical Sciences
|November 25, 2016
PubMed
Summary

This study refines a molecular diagnostic method for detecting beta-lactamase (bla) genes, crucial for combating antibiotic resistance. The improved method demonstrates high accuracy in identifying diverse bla genes in clinical samples.

Keywords:
Large-scale detectionMinimizing antibiotic resistanceMolecular diagnosisβ-Lactamase (bla) gene

More Related Videos

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

2.7K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

1.5K

Related Experiment Videos

Last Updated: Mar 11, 2026

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.6K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

2.7K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

1.5K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Antibiotic resistance, driven by beta-lactamase (bla) genes, poses a significant global health threat.
  • Existing molecular diagnostic methods for bla genes are limited in scope, failing to detect many clinically relevant variants.
  • Rapid and comprehensive detection of bla genes is essential for effective infection control and treatment strategies.

Purpose of the Study:

  • To evaluate a refined, efficient large-scale bla gene detection method (large-scale blaFinder) using clinical isolates.
  • To assess the method's accuracy and sensitivity in identifying a broad spectrum of bla genes.
  • To address the limitations of current diagnostic tools for beta-lactamase resistance.

Main Methods:

  • Development and application of the efficient large-scale blaFinder method, capable of detecting 1,352 bla gene types.
  • Testing the refined method on clinical isolates provided by International Health Management Associates, Inc.
  • Blinded interpretation of results by researchers unfamiliar with the isolates' genetic information.

Main Results:

  • The large-scale blaFinder successfully detected nearly all bla genes present in the clinical isolates, demonstrating high accuracy.
  • A single discrepancy was observed where a blaDHA-1 gene, detected by multiplex PCR, was not identified by the large-scale blaFinder in an Escherichia coli isolate.
  • This exception was attributed to the truncation of the blaDHA-1 gene.

Conclusions:

  • The refined large-scale blaFinder method offers a highly sensitive and specific tool for detecting a wide array of beta-lactamase genes.
  • Gene truncation, as exemplified by blaDHA-1, can be a factor limiting the detection capabilities of molecular diagnostic assays.
  • Further optimization of molecular methods is necessary to ensure comprehensive detection of all clinically relevant bla gene variants, including truncated forms.