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Related Experiment Video

Updated: Mar 11, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

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In vitro suppression of two different stop codons.

Eden Ozer¹, Yonatan Chemla¹, Orr Schlesinger¹

¹Department of Life Sciences and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, P. O. Box 653, Beer-Sheva, 84105, Israel.

Biotechnology and Bioengineering

|November 25, 2016

Summary

This summary is machine-generated.

Researchers successfully incorporated two distinct unnatural amino acids into a single protein using a cell-free system. This expands the genetic code and protein engineering capabilities beyond previous in vivo limitations.

Keywords:

Förster resonance energy transfer cell-free protein synthesis dual genetic code expansion orthogonal translation systems

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Last Updated: Mar 11, 2026

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Published on: March 7, 2019

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Published on: August 11, 2010

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Proteins are essential biomolecules constructed from 20 natural amino acids.
Unnatural amino acids are modified building blocks with unique properties.
Incorporating unnatural amino acids expands the genetic code and protein functionalities.

Purpose of the Study:

To develop a method for incorporating two different unnatural amino acids into a single protein.
To utilize a cell-free protein synthesis system for this purpose.
To overcome limitations of previous in vivo systems.

Main Methods:

Utilized a cell-free protein synthesis system.
Engineered the system to respond to two distinct stop codons.
Incorporated two different unnatural amino acids simultaneously.

Main Results:

Successfully synthesized a protein with two different unnatural amino acids.
Demonstrated the incorporation in response to two unique stop codons.
Achieved this using a cell-free system, a novel approach.

Conclusions:

Cell-free protein synthesis enables the efficient incorporation of multiple unnatural amino acids.
This technique expands the possibilities for protein engineering and functional studies.
Offers a powerful tool for manipulating and creating novel proteins.