Altered CO2 sensitivity of connexin26 mutant hemichannels in vitro

Connexin26 (Cx26) mutations underlie human pathologies ranging from hearing loss to keratitis ichthyosis deafness (KID) syndrome. Cx26 hemichannels are directly gated by CO₂ and contribute to the chemosensory regulation of breathing. The KID syndrome mutation A88V is insensitive to CO₂, and has a dominant negative action on the CO₂ sensitivity of Cx26^WT hemichannels, and reduces respiratory drive in humans. We have now examined the effect of further human mutations of Cx26 on its sensitivity to CO₂ : Mutated Cx26 subunits, carrying one of A88S, N14K, N14Y, M34T, or V84L, were transiently expressed in HeLa cells. The CO₂-dependence of hemichannel activity, and their ability to exert dominant negative actions on cells stably expressing Cx26^WT, was quantified by a dye-loading assay. The KID syndrome mutation, N14K, abolished the sensitivity of Cx26 to CO₂ Both N14Y and N14K exerted a powerful dominant negative action on the CO₂ sensitivity of Cx26^WT None of the other mutations (all recessive) had a dominant negative action. A88S shifted the affinity of Cx26 to slightly higher levels without reducing its ability to fully open to CO₂ M34T did not change the affinity of Cx26 for CO₂ but reduced its ability to open in response to CO₂ V84L had no effect on the CO₂-sensitivity of Cx26. Some pathological mutations of Cx26 can therefore alter the CO₂ sensitivity of Cx26 hemichannels. The loss of CO₂ sensitivity could contribute to pathology and consequent reduced respiratory drive could be an unrecognized comorbidity of these pathologies.