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Published on: March 31, 2021
A Potential Biofilm Metabolite Signature for Caries Activity - A Pilot Clinical Study
F Zandona1, H A Soini2, M V Novotny2
1Department of Operative Dentistry, University of North Carolina School of Dentistry at Chapel Hill, Chapel Hill, USA.
Background:
This study's aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses.
Methods:
Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS.
Results:
Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites.
Conclusions:
This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.
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