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Methods to Study Antimicrobial Resistance in Campylobacter jejuni
Orhan Sahin1,2, Zhangqi Shen1, Qijing Zhang3
1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 1116 Veterinary Medicine Complex, 1600 S. 16th Street, Ames, IA, 50011, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 26, 2016
Summary
Campylobacter jejuni causes widespread foodborne illness and antibiotic resistance. This chapter details molecular methods to detect resistance, aiding clinical treatment and monitoring resistant C. jejuni strains.
Area of Science:
- Microbiology
- Molecular Biology
- Public Health
Background:
- Campylobacter jejuni is a primary cause of bacterial foodborne gastroenteritis globally.
- Increasing antibiotic resistance in C. jejuni poses significant public health challenges.
- Both phenotypic and genotypic methods are available for detecting antibiotic resistance.
Purpose of the Study:
- To describe commonly used molecular biology methods for detecting antibiotic resistance in C. jejuni.
- To provide guidance for clinical and research settings.
- To support effective clinical therapy and surveillance of antibiotic resistance.
Main Methods:
- Focus on molecular biology techniques for genotypic detection.
- Description of commonly employed methods for antibiotic resistance gene identification.
- Application in both clinical diagnostics and research surveillance.
Main Results:
- Detailed explanation of molecular methods for detecting resistance.
- Highlighting the utility of these methods in various settings.
- Facilitating accurate identification of resistant C. jejuni.
Conclusions:
- Molecular methods are crucial for detecting antibiotic resistance in C. jejuni.
- These techniques are vital for guiding treatment decisions.
- Essential for monitoring the emergence and spread of antibiotic-resistant C. jejuni strains.
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