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Methods to Assess the Direct Interaction of C. jejuni with Mucins
Marguerite Clyne1, Gina Duggan1, Julie Naughton2
1Conway Institute, School of Medicineand Medical Science, University College Dublin, Dublin, Ireland.
Abstract:
Studies of the interaction of bacteria with mucus-secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far Western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed using biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high-throughput manner.
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