Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

1.5K
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
1.5K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

3.2K
3.2K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

2.8K
2.8K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

9.2K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
9.2K
Preparation of 1° Amines: Hofmann and Curtius Rearrangement Mechanism01:26

Preparation of 1° Amines: Hofmann and Curtius Rearrangement Mechanism

4.2K
The Hofmann and Curtius rearrangement reactions can be applied to synthesize primary amines from carboxylic acid derivatives such as amides and acyl azides. In the Hofmann rearrangement, a primary amide undergoes deprotonation in the presence of a base, followed by halogenation to generate an N-haloamide. A second proton abstraction produces a stabilized anionic species, which rearranges to an isocyanate intermediate via an alkyl group migration from the carbonyl carbon to the neighboring...
4.2K
Molecules with Multiple Chiral Centers02:25

Molecules with Multiple Chiral Centers

16.1K
Molecules that possess multiple chiral centers can afford a large number of stereoisomers. For instance, while some molecules like 2-butanol have one chiral center, defined as a tetrahedral carbon atom with four different substituents attached, several molecules like butane-2,3-diol have multiple chiral centers. A simple formula to predict the number of stereoisomers possible for a molecule with n chiral centers is 2n. However, there can be a lower number where some of the stereoisomers are...
16.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression profiles for macrophage alternative activation genes in AD and in mouse models of AD.

Journal of neuroinflammationยท2006
See all related articles

Related Experiment Videos

A new trick for an old lipid.

Hayley Sharpe1

  • 1Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.

Elife
|November 26, 2016
PubMed
Summary

Cholesterol regulates the Hedgehog signaling pathway by binding to cell surface receptors. This interaction influences cellular processes, highlighting cholesterol's role in developmental biology.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Developmental Biology

Background:

  • Cholesterol is a vital lipid with diverse cellular functions.
  • The Hedgehog signaling pathway is crucial for embryonic development and tissue homeostasis.
  • Aberrant Hedgehog signaling is implicated in various cancers.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which cholesterol interacts with the Hedgehog signaling pathway.
  • To investigate the role of cell surface receptors in mediating cholesterol's effects on Hedgehog signaling.

Main Methods:

  • Cell-based assays were employed to study cholesterol-Hedgehog pathway interactions.
  • Specific cell surface receptors were targeted to assess their role in mediating the observed effects.
Keywords:
G-protein coupled receptorHedgehog signalingbiochemistrycholesteroldevelopmental biologyhumanmousestem cells

Related Experiment Videos

  • Biochemical and molecular biology techniques were utilized to analyze pathway components.

    • Main Results:

    • Cholesterol directly binds to a specific cell surface receptor involved in Hedgehog pathway activation.
    • This binding event modulates receptor activity and downstream signaling.
    • The interaction between cholesterol and the receptor influences cellular responses regulated by Hedgehog signaling.

    Conclusions:

    • Cholesterol acts as a direct regulator of the Hedgehog signaling pathway through cell surface receptor binding.
    • This finding reveals a novel mechanism controlling a key developmental pathway.
    • Targeting this cholesterol-receptor interaction may offer therapeutic strategies for diseases associated with Hedgehog pathway dysregulation.