Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

NMR Spectroscopy: Spin–Spin Coupling

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...

2D NMR: Overview of Heteronuclear Correlation Techniques

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...

¹H NMR: Interpreting Distorted and Overlapping Signals

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...

2D NMR: Overview of Homonuclear Correlation Techniques

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

Two NMR-active nuclei bonded to a central atom can be involved in geminal or two-bond coupling. Geminal coupling is commonly seen between diastereotopic protons in chiral molecules and unsymmetrical alkenes, among others.
The central atom need not be NMR-active because its electrons are affected by the electron polarization of the spin-active atoms. However, spin information is transmitted less effectively than in one-bond coupling, and 2J values are usually weaker than 1J values. The energy of...

Colors and Magnetism

Colors and Magnetism

Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ligilactobacillus murinus modulates gut microbiota-derived 5-hydroxyindole to inhibit pseudorabies virus infection via activation of the aryl hydrocarbon receptor.

NPJ biofilms and microbiomes·2026

Same author

Protective Effect of <i>Escherichia coli Nissle 1917</i> on <i>Salmonella typhimurium</i> Infection by Regulating Intestinal Flora.

Microorganisms·2026

Same author

Context-dependent functions of ALKBH5: a mechanistic framework linking cellular stress responses, immune regulation, viral infection, and therapeutic vulnerabilities.

Frontiers in immunology·2026

Same author

Transcriptomic Analysis of Fermented Chinese Chive Selectively Attenuating Deoxynivalenol-Induced Ovarian Toxicity in Mice.

Antioxidants (Basel, Switzerland)·2026

Same author

Engineered <i>Lactobacillus casei</i> targets the IgT-pIgR axis to confer mucosal protection against <i>Aeromonas veronii</i> in snakehead (<i>Channa argus</i>).

Frontiers in immunology·2026

Same author

Evaluation of immune protective efficacy of recombinant adenovirus vector vaccine containing RBS of influenza virus subtype H1N1.

Microbial pathogenesis·2026

Search research articles

Related Experiment Video

Updated: Mar 11, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Multiple correlations between spin crossover and fluorescence in a dinuclear compound.

Chun-Feng Wang¹, Ming-Jun Sun², Qi-Jie Guo²

¹State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, People's Republic of China. zxcao@xmu.edu.cn taojun@xmu.edu.cn and Key Laboratory of Cluster Science of Ministry of Education, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488, People's Republic of China. taojun@bit.edu.cn.

Chemical Communications (Cambridge, England)

|November 26, 2016

Summary

This summary is machine-generated.

This study reveals strong links between spin crossover and fluorescence in a dinuclear iron compound. Energy transfer processes involving iron and ligands are key to these observed correlations.

More Related Videos

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Related Experiment Videos

Last Updated: Mar 11, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Area of Science:

Coordination Chemistry
Materials Science
Photophysics

Background:

Spin crossover (SCO) materials exhibit distinct magnetic and optical properties.
Fluorescence in dinuclear compounds can arise from complex charge transfer transitions.
Understanding structure-property relationships is crucial for designing functional materials.

Purpose of the Study:

To investigate the relationship between spin crossover and fluorescence in a specific dinuclear iron compound.
To elucidate the charge transfer pathways responsible for fluorescence.
To explore the role of energy transfer in correlating spin crossover and fluorescence.

Main Methods:

Synthesis and characterization of the dinuclear iron compound.
Spectroscopic analysis (UV-Vis, fluorescence spectroscopy).
Variable temperature magnetic susceptibility measurements to probe spin crossover behavior.

Main Results:

Established multiple correlations between spin crossover and fluorescence phenomena.
Identified fluorescence originating from d(Fe) + π(SCN) to π*(ligand) charge transfer.
Demonstrated correlation arising from ligand to Fe(ii) energy transfer.

Conclusions:

The dinuclear compound exhibits coupled spin crossover and fluorescence behavior.
Ligand-to-metal energy transfer plays a significant role in the observed phenomena.
These findings offer insights into the design of multifunctional coordination compounds.