Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inflammatory myofibroblasts reprogram neutrophil fate to drive chronic inflammation in peri-implantitis.

International journal of oral science·2026
Same author

Tibial periosteal distraction as an adjunct to percutaneous transluminal angioplasty for diabetic foot ulcers complicated by peripheral arterial disease/chronic limb-threatening ischemia: a retrospective cohort study.

Journal of orthopaedic surgery and research·2026
Same author

Fecal microbiota transplantation from different pig breeds alters fat deposition and gut microbiota in mice.

Applied microbiology and biotechnology·2026
Same author

YTHDC1 recognizes METTL16-dependent m<sup>6</sup>A on caRNAs and coordinates cotranscriptional splicing.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The internal representation of fractions: component, holistic or hybrid?

Frontiers in neuroscience·2026
Same author

The m6Am methyltransferase PCIF1 promotes osteogenic differentiation of mesenchymal stem cells through stabilization of Wnt-related transcripts.

PLoS biology·2026

Related Experiment Video

Updated: Mar 11, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

11.1K

Multifunctional nanoparticles for protein detections in thin channels.

Hweiyan Tsai1, Weimin Lin2, Mingchieh Chuang2

  • 1School of Medical Applied Chemistry, Chung Shan Medical University, Taichung 402, Taiwan; Department of Medical Education, Chung Shan Medical University Hospital, Taichung 402, Taiwan.

Biosensors & Bioelectronics
|November 26, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a novel magnetic immunoassay for detecting two cancer biomarkers simultaneously using multifunctional nanoparticles. This method offers enhanced sensitivity and wider linear ranges compared to traditional assays.

Keywords:
Biofunctional nanoparticlesMagnetic immunoassayThin channel

More Related Videos

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

14.4K
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
08:34

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

10.6K

Related Experiment Videos

Last Updated: Mar 11, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

11.1K
Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

14.4K
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
08:34

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

10.6K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Simultaneous protein detection is crucial for accurate disease diagnosis.
  • Existing immunoassay methods often lack sensitivity and have narrow detection ranges.
  • Developing rapid, sensitive, and selective multiplexed biomarker detection is a significant challenge.

Purpose of the Study:

  • To develop a method for simultaneous detection of two proteins using multifunctional nanoparticles and a magnetic immunoassay.
  • To evaluate the performance of the proposed method using alpha-fetoprotein (AFP) and cancer antigen 125 (CA125) as model biomarkers.
  • To compare the proposed method with traditional enzyme-linked immunosorbent assay (ELISA).

Main Methods:

  • Utilized biofunctional magnetic graphene quantum dots (GQDs) for nanoparticle deposition and blue emission.
  • Employed two types of biofunctional quantum dots (QDs) for specific protein detection with orange and green emissions.
  • Performed simultaneous detection of AFP and CA125 in thin channels using a magnetic immunoassay.
  • Quantified protein levels based on nanoparticle deposition and fluorescence emission.

Main Results:

  • Achieved low detection limits (0.06 pg/mL AFP, 0.001 U/mL CA125) and wide linear ranges (0.2 pg/mL–0.68 ng/mL AFP, 0.003–25 U/mL CA125).
  • Demonstrated comparable performance to single protein detection, indicating minimal cross-interference.
  • Showed less than 12% difference compared to ELISA for serum sample measurements.
  • Exhibited superior sensitivity and wider linear ranges than conventional ELISA and other immunoassays.

Conclusions:

  • The proposed multifunctional nanoparticle-based magnetic immunoassay enables sensitive and selective simultaneous detection of two protein biomarkers.
  • This method offers significant advantages in terms of speed, sensitivity, selectivity, and throughput over existing techniques.
  • The developed assay shows great potential for clinical diagnostics and biomarker discovery.