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Related Experiment Video

Updated: Mar 11, 2026

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

Novel multi-functional fluid flow device for studying cellular mechanotransduction.

James S Lyons¹, Shama R Iyer¹, Richard M Lovering¹

¹Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Journal of Biomechanics

|November 27, 2016

Summary

Researchers developed a new, low-cost fluid flow device to study cell mechanotransduction. This tool enables efficient investigation of how cells respond to mechanical forces, aiding in understanding related diseases.

Keywords:

Bone cells Fluid shear stress Intracellular calcium Mechanotransduction Osteoblast Osteocyte Signal transduction

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Area of Science:

Biotechnology
Cell Biology
Biomedical Engineering

Background:

Cells dynamically respond to mechanical stimuli through complex mechanotransduction pathways.
Dysfunctional mechanotransduction is linked to various diseases, highlighting the need for effective research tools.
Mechanical stimulation of cells in culture, often via fluid flow, is crucial for studying these responses.

Purpose of the Study:

To introduce a novel, multifunctional fluid flow device for in vitro cell mechanotransduction studies.
To provide a cost-effective and versatile alternative to existing cell stimulation systems.
To validate the device's efficacy in generating physiologically relevant mechanical conditions.

Main Methods:

Development of a novel fluid flow device compatible with standard cell culture plates and pumps.
Validation using UMR-106 osteoblast-like cells subjected to fluid flow.
Comparison with a commercial laminar shear stress system to measure live cell calcium influx.
Assessment of phospho-ERK activation as a molecular readout of mechanotransduction.

Main Results:

The device successfully exposed cells to controlled fluid flow, mimicking physiological conditions.
Live cell calcium influx was effectively tracked in response to fluid flow.
Fluid flow-induced activation of phospho-ERK was confirmed, aligning with established mechanotransduction responses.
The device demonstrated comparable biological responses to a commercial system.

Conclusions:

The developed fluid flow device offers a low-cost, multifunctional, and efficient method for studying cellular mechanotransduction in vitro.
This tool facilitates the investigation of cell responses to mechanical forces, relevant to understanding pathologies.
The device integrates seamlessly with common laboratory equipment, enhancing accessibility for researchers.