Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Interleukin-10 enhances IgG galactosylation and sialylation.

Biomarker research·2026

Same author

Glycosylation profile of <i>Mycobacterium leprae</i>-specific antibodies associated with disseminated infection.

iScience·2026

Same author

Longitudinal Maternal IgG and IgA Glycosylation Profiles in Pregnancy Reveal Early Immune Alterations in Placenta-Related Complications: The Rotterdam Periconception Cohort.

Hypertension (Dallas, Tex. : 1979)·2026

Same author

Fc-fucosylation of Plasmodium falciparum-specific IgG varies with antigen and immunization.

The Journal of infectious diseases·2026

Same author

Detection of highly hydrophilic glycopeptides in reverse-phase liquid chromatography-mass spectrometry after TMTPro Zero labeling.

Carbohydrate polymers·2026

Same author

Type 1 LacNAc and LacdiNAc on N-glycans as potential molecular biomarkers of human melanoma cells.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Mar 11, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Reversed-phase separation methods for glycan analysis.

Gerda C M Vreeker^1,2, Manfred Wuhrer^3,4

¹Center for Proteomics and Metabolomics, Leiden University Medical Center, PO Box 9600, 2300 RC, Leiden, The Netherlands.

Analytical and Bioanalytical Chemistry

|November 27, 2016

Summary

Reversed-phase chromatography enables glycan isomer separation by tagging glycans with hydrophobic molecules. This method

Keywords:

Glycan Liquid chromatography Reversed phase Separation

More Related Videos

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Published on: May 22, 2016

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Related Experiment Videos

Last Updated: Mar 11, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Published on: May 22, 2016

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Area of Science:

Analytical Chemistry
Biochemistry
Glycobiology

Background:

Glycan analysis is crucial for understanding biological processes.
Reversed-phase chromatography (RPC) is widely used for glycan separation.
Glycan derivatization with hydrophobic tags is necessary for retention in RPC.

Approach:

Investigating the impact of hydrophobic tags and glycan structure on retention and elution order in RPC.
Exploring the role of specific monosaccharides, sialic acids, and fucoses in glycan retention.
Evaluating RPC's capability for separating isomeric and isobaric glycans, especially when coupled with mass spectrometry.

Key Points:

Glycan retention in RPC depends on tag hydrophobicity and glycan structure, including monosaccharide position and linkage.
Separation of complex glycan mixtures can be challenging with UV/fluorescence detection but is improved with mass spectrometry.
RPC can achieve separation of isomeric and isobaric glycans under optimized conditions.

Conclusions:

RPC is a versatile and accessible platform for glycan analysis, offering isomer separation capabilities.
While other methods like HILIC and porous graphitized carbon chromatography exist, RPC's widespread use makes it attractive.
Further research is needed to fully understand the influence of sialic acids and fucoses on glycan retention in RPC.