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Final irrigation protocols may affect intraradicular dentin ultrastructure.

Márcia Helena Wagner¹, Ricardo Abreu da Rosa¹, José Antonio Poli de Figueiredo²

¹Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.

Clinical Oral Investigations

|November 27, 2016

Summary

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This summary is machine-generated.

Sodium hypochlorite (NaOCl) irrigation alters root dentin collagen. When combined with EDTA and/or passive ultrasonic irrigation (PUI), NaOCl causes significant peritubular and intertubular dentin erosion, impacting root canal treatment outcomes.

Area of Science:

Endodontics
Dental Materials Science
Microscopy Techniques

Background:

Root canal irrigation is crucial for disinfection but can affect dentin structure.
Understanding the ultrastructural effects of irrigants is vital for safe and effective endodontic treatment.

Purpose of the Study:

To investigate the impact of various irrigation protocols on root dentin ultrastructure.
To evaluate the combined effects of sodium hypochlorite (NaOCl), EDTA, and passive ultrasonic irrigation (PUI) on dentin collagen and peritubular dentin.

Main Methods:

Bovine incisors were treated with different irrigation solutions including distilled water, saline, EDTA, NaOCl, chlorhexidine (CHX), and combinations with PUI.
Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to analyze root dentin ultrastructure.

Keywords:

Chlorhexidine Dentine EDTA Endodontics Sodium hypochlorite

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Main Results:

NaOCl caused thinning of dentin collagen fibrils, an effect amplified by EDTA and/or PUI.
NaOCl combined with PUI resulted in significantly greater peritubular dentin erosion compared to other groups.
NaOCl with EDTA and NaOCl with EDTA and PUI also showed notable erosion.

Conclusions:

NaOCl induces ultrastructural changes in dentin collagen.
EDTA and PUI enhance the erosive effects of NaOCl on both peritubular and intertubular dentin.
Careful consideration of irrigant type, concentration, and application sequence is necessary to maintain dentin integrity during root canal treatment.