Related Experiment Video
Updated: Mar 11, 2026
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
Published on: May 18, 2021
Development of a novel engineered antibody targeting Neisseria species
Chih-Yueh Liu1, Chang-Ching Weng1, Chih-Hsiang Lin1
1Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan.
Objectives:
A Neissaria bacterial pilus sugar, bacillosamine, was synthesized and, for the first time, used as a probe to screen a single-chain variable fragment (scFv).
Results:
Four Neisseria, Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria sicca and Neisseria subflava, and two negative controls, Streptococcus pneumoniae and Escherichia coli, were tested through ELISA, immunostaining and gold nanoparticle immunological assay. All results indicated that the selected scFv is feasible for the specific detection of Neisseria species via the recognition of bacillosamine.
Conclusions:
The recombinant scFv could detect Neisseria strains at 106 CFU/ml.
More Related Videos
12:30Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77
Published on: August 17, 2015
07:04Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
Published on: January 24, 2025
