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The utility of direct specimen detection by Sanger sequencing in hospitalized pediatric patients
Kanokporn Mongkolrattanothai1, Jennifer Dien Bard2
1Division of Infectious Diseases, Department of Pediatrics, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033, USA.
Abstract:
Direct microbial DNA detection from clinical specimens by polymerase chain reaction and Sanger sequencing has been developed to address the innate limitations of traditional culture-based work-up. We report our institution's experience with direct specimen sequencing, its clinical utility, and barriers to effective clinical implementation.

