Related Experiment Video

Updated: Mar 11, 2026

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

8.0K

MiR-146a Regulates Inflammatory Infiltration by Macrophages in Polymyositis/Dermatomyositis by Targeting TRAF6 and

Yuanqin Yin1, Fei Li, Jing Shi

  • 1Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|November 28, 2016
PubMed
Abstract

Insights

MicroRNA-146a (miR-146a) plays a key role in polymyositis/dermatomyositis (PM/DM) by targeting TRAF6, influencing macrophage infiltration and the IL-17/ICAM-1 pathway. This finding offers new insights into PM/DM pathogenesis.

Area of Science:

  • Immunology
  • Molecular Biology
  • Rheumatology

Background:

  • Polymyositis/dermatomyositis (PM/DM) is characterized by inflammatory macrophage infiltration.
  • The precise molecular mechanisms driving this infiltration require further elucidation.

Purpose of the Study:

  • To investigate the role of microRNA-146a (miR-146a) in macrophage infiltration in PM/DM.
  • To explore the potential targeting of TNF receptor associated factor 6 (TRAF6) by miR-146a.
  • To examine the impact on the Interleukin-17 (IL-17)/Intercellular Adhesion Molecule 1 (ICAM-1) pathway.

Main Methods:

  • Analysis of muscle biopsies from PM/DM patients and healthy controls.
  • Establishment of a PM/DM rat model and macrophage isolation.
  • In vitro and in vivo treatments with miR-146a mimics/inhibitors, TRAF6 siRNAs, and pathway-specific antibodies.
  • Assessment of serum creatine phosphokinase (S-CK), CD163 expression, cell migration, and molecular targets (miR-146a, TRAF6, IL-17, ICAM-1) via ELISA, immunohistochemistry, transwell assays, RT-PCR, and western blot.

Main Results:

  • Elevated S-CK, TRAF6, IL-17, and ICAM-1 levels in PM/DM patients, reduced by conventional treatment.
  • miR-146a mimics and pathway inhibitors decreased IL-17 and ICAM-1; miR-146a inhibitors increased them.
  • TRAF6 siRNA counteracted the effects of miR-146a inhibitors, confirming TRAF6 involvement.
  • Luciferase reporter assay confirmed direct targeting of TRAF6 by miR-146a.

Conclusions:

  • MiR-146a regulates inflammatory macrophage infiltration in PM/DM.
  • This regulation occurs through targeting TRAF6 and modulating the IL-17/ICAM-1 pathway.
  • These findings highlight miR-146a as a potential therapeutic target in PM/DM.

More Related Videos

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin10:21

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Published on: September 12, 2019

7.8K
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

8.7K

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.0K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.6K
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
17.9K