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Inhibition of ERK/MAPK suppresses avian leukosis virus subgroup A and B replication

Manman Dai1, Min Feng2, Ming Liao1

  • 1College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, People's Republic of China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, People's Republic of China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, People's Republic of China.

Microbial Pathogenesis
|November 29, 2016
PubMed

Insights

The extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway is crucial for avian leukosis virus (ALV) replication. Inhibiting this pathway significantly reduces ALV-A and ALV-B viral replication, suggesting new therapeutic targets.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Signaling

Background:

  • The extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway is known to influence avian leukosis virus subgroup J (ALV-J) replication and tumor formation.
  • The role of ERK/MAPK signaling in the replication of avian leukosis virus subgroups A (ALV-A) and B (ALV-B) remains uncharacterized.

Purpose of the Study:

  • To investigate the role of the ERK/MAPK signaling pathway in the replication of ALV-A and ALV-B.
  • To determine if ERK/MAPK signaling is a potential therapeutic target for ALV infections.

Main Methods:

  • Construction and recovery of a recombinant ALV-A strain (GD13-1).
  • Assessment of ERK2 activation in primary chicken embryo fibroblast (CEF) cells upon infection with ALV subgroups J, A, and B.
  • Evaluation of ALV-A and ALV-B replication following ERK/MAPK pathway inhibition.

Main Results:

  • All tested ALV subgroups (J, A, and B) induced ERK2 activation in primary CEF cells.
  • Inhibition of the ERK/MAPK pathway significantly suppressed ALV-A and ALV-B replication.
  • Viral transcription and protein production were markedly reduced in ALV-A and ALV-B infected cells upon ERK/MAPK inhibition.

Conclusions:

  • ERK/MAPK signaling plays a significant role in the replication of avian leukosis virus subgroups A and B.
  • The ERK/MAPK pathway represents a potential novel drug target for therapeutic strategies against ALV infections.
Keywords:
ALV-AALV-BERK/MAPK pathwayVirus replication

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