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Updated: Mar 11, 2026
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Rapid identification of Robinsoniella peoriensis using specific 16S rRNA gene PCR primers
Terence R Whitehead1, Christelle Anoma2, Richard W McLaughlin2
1Bioenergy Research Unit, National Center for Agricultural Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA.
Abstract:
Robinsoniella peoriensis is a Gram-positive, spore-forming anaerobic bacterium initially isolated and characterized from swine manure and feces. Since then strains of this species have been identified from a variety of mammalian and other GI tracts. More recently strains of this species have been isolated from a plethora of human infections. Therefore, it is of great interest to develop methods to rapidly identify this microorganism in the medical and other laboratories. This report describes the use of PCR primers targeting the 16S rRNA gene of R. peoriensis to identify strains of this bacterium.
Insights
This study developed a rapid PCR method to identify Robinsoniella peoriensis, a bacterium increasingly found in human infections. This technique aids quick detection in clinical and laboratory settings.
Area of Science:
- Microbiology
- Molecular Biology
- Clinical Diagnostics
Background:
- Robinsoniella peoriensis is a Gram-positive, anaerobic bacterium.
- Initially found in swine, it is now increasingly identified in human infections.
- Rapid identification methods are needed for clinical and laboratory settings.
Purpose of the Study:
- To develop and describe a rapid method for identifying Robinsoniella peoriensis.
Main Methods:
- Utilized Polymerase Chain Reaction (PCR) primers.
- Targeted the 16S rRNA gene specific to R. peoriensis.
Main Results:
- Successfully developed PCR primers for R. peoriensis identification.
- Demonstrated the utility of PCR for rapid detection of the bacterium.
Conclusions:
- PCR targeting the 16S rRNA gene provides a rapid and effective method for identifying Robinsoniella peoriensis.
- This method can aid in the diagnosis and management of infections caused by this bacterium.
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