Related Experiment Video
Updated: Mar 11, 2026
Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey
Published on: October 14, 2014
Two-headed mutants of the lamprey, a basal vertebrate
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572 Japan ; Nobel Institute for Neurophysiology, Department of Neuroscience, Karolinska Institutet, SE-17177, Stockholm, Sweden.
Background:
This is the first report of two-headed (bicephaly) lamprey twins. Although lampreys sit at a crucial phylogenetic position, there are only a few reports on their teratology and developmental abnormalities.
Results:
Two-headed mutants were obtained by artificial fertilization in the laboratory as spontaneous occurrences. All mutants were derived from single fertilizations using single male and female gametes, suggestive of a genetic background. The anterio-posterior position of the axonal bifurcation and symmetricity varied in each mutant. Other malformations were coincidently observed, including pericardial edema, yolk sac edema and axial bending. Asymmetrical (lateral- branched) mutants displayed more severe abnormalities in the cranial nerves than symmetrical mutants.
Conclusion:
Two-headed mutants of the lamprey are described. These mutants have similar malformations to dorsal blastopore lip-transplanted lamprey embryos, suggesting that they could be generated by a disorder in head-organizing activity.
More Related Videos
07:03A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey Petromyzon marinus
Published on: June 6, 2014
09:31Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
Published on: January 12, 2015