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Establishment of Rat Models Mimicking Gender-affirming Hormone Therapies

Establishment of Rat Models Mimicking Gender-affirming Hormone Therapies

Published on: January 10, 2025

Melamine negatively affects testosterone synthesis in mice.

Jiarui Sun¹, Yinan Cao¹, Xinchen Zhang¹

¹College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Research in Veterinary Science

|November 29, 2016

Summary

Melamine exposure damages testes and sperm by reducing testosterone levels. This occurs through down-regulation of key enzymes and Leydig cell reduction, impacting male reproductive health.

Keywords:

Leydig cell Melamine Steroidogenesis Testis Testosterone

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Area of Science:

Reproductive Toxicology
Endocrinology
Environmental Health

Background:

Melamine is a known testicular toxicant.
Testosterone is crucial for male reproductive functions.
The impact of melamine on testosterone synthesis is not well understood.

Purpose of the Study:

To investigate the effects of melamine on testosterone synthesis in mice.
To elucidate the mechanisms underlying melamine-induced testicular damage.

Main Methods:

Mice were orally administered melamine (2, 10, or 50 mg/kg) for 28 days.
Evaluated testicular structure, sperm parameters, and serum/testicular testosterone levels.
Assessed expression of steroidogenic enzymes (StAR, P450scc, 17β-HSD) and Leydig cell count.

Main Results:

Melamine induced seminiferous tubule damage, germ cell necrosis, and sperm abnormalities.
A dose-dependent decrease in serum testosterone and testicular StAR, P450scc, and 17β-HSD levels was observed.
Melamine exposure reduced Leydig cell numbers.

Conclusions:

Melamine reduces testosterone levels by down-regulating steroidogenic enzyme expression and decreasing Leydig cell count.
These effects contribute to melamine-induced testicular damage and impaired spermatogenesis.
Melamine poses a risk to male reproductive health.