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MiRNA-378 controls cell proliferation in rabbit umbilical cord mesenymal stem cells
1College of Wildlife Resources, Northeast Forestry University, Harbin, China.
Abstract:
Mesenchymal stem cells (MSC) are known to have the ability to differentiate into various lineages of mesenchymal tissue. They are widely distributed in a variety of tissues in the body and are also present in the foetal environment. MicroRNAs (miRNAs) are a fundamental class of biological molecules that play a crucial role in development. In this study, microRNA-378 and its predicted target (Sufu, suppressor of fused homolog) were used to study proliferation of rabbit umbilical cord mesenymal stem cells, luciferase reporter assay was used to assess and confirm the binding sequence of 3'untranslated region between microRNA-378 and Sufu. The results showed that miRNA-378 overexpression reduced Sufu gene, and promoted cell proliferation of rabbit umbilical cord mesenymal stem cells via cell viability and BrdU testing, which molecular mechanisms were down-regulation of apoptosis genes after miRNA-378 overexpression. In conclusion, our research shows that the microRNA-378 promoted the cell proliferation of rabbit umbilical cord mesenymal stem cells, and this mechanism was miRNA-378 reduced the expression of Sufu, increased cell proliferation.
Insights
MicroRNA-378 promotes rabbit umbilical cord mesenchymal stem cell proliferation by downregulating Sufu. This mechanism enhances cell viability and reduces apoptosis, offering insights into stem cell regulation.
Area of Science:
- Stem cell biology
- Molecular biology
- Gene regulation
Background:
- Mesenchymal stem cells (MSCs) differentiate into various mesenchymal lineages and are found in diverse tissues and fetal environments.
- MicroRNAs (miRNAs) are key regulators of biological development.
Purpose of the Study:
- To investigate the role of microRNA-378 (miRNA-378) in regulating the proliferation of rabbit umbilical cord mesenchymal stem cells (RUC-MSCs).
- To elucidate the molecular mechanism by which miRNA-378 influences RUC-MSC proliferation, focusing on its interaction with the Sufu gene.
Main Methods:
- Utilized luciferase reporter assays to confirm the binding interaction between miRNA-378 and the 3' untranslated region of the Sufu gene.
- Overexpressed miRNA-378 in RUC-MSCs and assessed its impact on cell proliferation using cell viability and BrdU assays.
- Analyzed the expression levels of apoptosis-related genes following miRNA-378 overexpression.
Main Results:
- Confirmed that miRNA-378 directly targets and reduces the expression of the Sufu gene in RUC-MSCs.
- Demonstrated that miRNA-378 overexpression significantly promotes RUC-MSC proliferation, evidenced by increased cell viability and BrdU incorporation.
- Observed a downregulation of apoptosis genes in RUC-MSCs after miRNA-378 overexpression, suggesting an anti-apoptotic effect.
Conclusions:
- MicroRNA-378 plays a crucial role in promoting the proliferation of rabbit umbilical cord mesenchymal stem cells.
- The mechanism involves the downregulation of Sufu gene expression by miRNA-378, leading to enhanced cell proliferation and reduced apoptosis.
