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microRNA-145 regulates the RLR signaling pathway in miiuy croaker after poly(I:C) stimulation via targeting MDA5

Jingjing Han1, Yuena Sun1, Weihua Song1

  • 1Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.

Developmental and Comparative Immunology
|November 30, 2016
PubMed

Insights

MicroRNAs regulate fish immunity by degrading messenger RNAs. MicroRNA-145-5p targets MDA5, impacting the RIG-I-like receptor signaling pathway in miiuy croaker.

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression.
  • The RIG-I-like receptor (RLR) pathway is crucial for innate immunity.
  • Understanding miRNA regulation of RLR signaling is vital for fish health.

Purpose of the Study:

  • To investigate the role of microRNA-145-5p (miR-145-5p) in regulating the RLR signaling pathway in miiuy croaker.
  • To identify and validate the target gene of miR-145-5p within this pathway.

Main Methods:

  • Bioinformatic prediction of miRNA-target interactions.
  • Luciferase reporter assays to confirm target gene binding.
  • Quantitative analysis of gene and miRNA expression in fish tissues.

Main Results:

  • MDA5 was identified as a direct target of miR-145-5p in miiuy croaker.
  • miR-145-5p effectively inhibited MDA5 expression in a dose- and time-dependent manner.
  • Expression patterns suggested miR-145-5p regulates MDA5 mRNA levels in vivo.

Conclusions:

  • miR-145-5p regulates the RLR signaling pathway by degrading MDA5 mRNA in miiuy croaker.
  • This study enhances understanding of miRNA-mediated immune responses in fish.
  • Findings provide a foundation for exploring complex miRNA regulatory networks in fish immunity.
Keywords:
MDA5MicroRNAsNegatively regulateRLRsmicroRNA-145

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