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Long Noncoding RNA-LET Suppresses Tumor Growth and EMT in Lung Adenocarcinoma

Bin Liu1, Chun-Feng Pan1, Zhi-Cheng He1

  • 1Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Biomed Research International
|November 30, 2016
PubMed

Insights

Long non-coding RNA-LET (lncRNA-LET) acts as a tumor suppressor in lung adenocarcinoma (LAC). Downregulation of lncRNA-LET promotes LAC progression, while its overexpression inhibits cancer growth by affecting EMT and the Wnt/β-catenin pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • lncRNA-LET has been previously identified as downregulated with antitumor effects in various cancers.

Purpose of the Study:

  • To investigate the specific role and mechanism of lncRNA-LET in the progression of lung adenocarcinoma (LAC).

Main Methods:

  • Reverse Transcription Polymerase Chain Reaction (RT-PCR) was used to analyze lncRNA-LET expression in LAC tissues.
  • Functional assays were performed to assess the effects of lncRNA-LET knockdown and overexpression on LAC cell proliferation, invasion, and migration.
  • The impact of lncRNA-LET on Epithelial-Mesenchymal Transition (EMT) and the Wnt/β-catenin pathway was evaluated.

Main Results:

  • Frequent downregulation of lncRNA-LET was observed in LAC tissues and correlated with clinicopathologic factors.
  • lncRNA-LET knockdown accelerated LAC cell proliferation, invasion, and migration.
  • Overexpression of lncRNA-LET suppressed LAC cell proliferation, invasion, and migration, indicating an antitumor role.
  • lncRNA-LET was found to inhibit EMT and negatively regulate the Wnt/β-catenin pathway.

Conclusions:

  • lncRNA-LET functions as a tumor suppressor in lung adenocarcinoma.
  • lncRNA-LET inhibits LAC progression by suppressing EMT and the Wnt/β-catenin pathway.
  • lncRNA-LET represents a potential therapeutic target for lung adenocarcinoma treatment.

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