Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
DNA Microarrays02:34

DNA Microarrays

21.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular adaptation of sweet potato to Martian soil simulant: insights from mRNA and long non-coding RNA analyses.

Journal of advanced research·2026
Same author

Natural variation in the ZmPIMT1 promoter enhances seed aging tolerance by regulating PABP2 repair in maize.

The Plant cell·2025
Same author

Revisiting CPSF30-mediated alternative polyadenylation in Arabidopsis thaliana.

PloS one·2025
Same author

Nucleotide-resolution Mapping of RNA N6-Methyladenosine (m6A) modifications and comprehensive analysis of global polyadenylation events in mRNA 3' end processing in malaria pathogen <i>Plasmodium falciparum</i>.

bioRxiv : the preprint server for biology·2025
Same author

A genomics learning framework for undergraduates.

PloS one·2025
Same author

Intraspecific variability of rice root knot nematodes across diverse agroecosystems for sustainable management.

Scientific reports·2024

Related Experiment Video

Updated: Mar 11, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

23.8K

De novo Transcriptome Assembly and Dynamic Spatial Gene Expression Analysis in Red Clover.

Manohar Chakrabarti, Randy D Dinkins, Arthur G Hunt

    The Plant Genome
    |November 30, 2016
    PubMed
    Summary

    This study presents a new red clover transcriptome assembly, offering valuable genomic resources. This resource aids in identifying key genes for improved livestock feed and understanding plant development.

    More Related Videos

    Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
    08:33

    Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

    Published on: August 5, 2020

    8.8K
    Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
    10:40

    Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

    Published on: December 22, 2017

    11.0K

    Related Experiment Videos

    Last Updated: Mar 11, 2026

    Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
    10:27

    Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

    Published on: July 30, 2011

    23.8K
    Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
    08:33

    Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

    Published on: August 5, 2020

    8.8K
    Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
    10:40

    Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

    Published on: December 22, 2017

    11.0K

    Area of Science:

    • Plant genomics
    • Agricultural science
    • Molecular biology

    Background:

    • Red clover (Trifolium pratense) is a vital cool-season forage legume, crucial for livestock feed due to its high protein and digestibility.
    • Despite its agricultural importance, genomic resources for red clover are currently limited.
    • This study addresses the scarcity of genomic data for red clover.

    Purpose of the Study:

    • To construct a de novo transcriptome assembly for red clover.
    • To identify genes, particularly transcription factors, and analyze gene expression patterns.
    • To provide a foundation for future research in red clover development and biotechnology.

    Main Methods:

    • A de novo transcriptome assembly was created from various red clover tissue types.
    • The assembly was annotated, and transcription factors were identified genome-wide.
    • Comparative genomics with other legumes and gene expression analysis across tissues were performed.

    Main Results:

    • The red clover transcriptome assembly comprises 37,565 contigs (N50: 1707 bp).
    • High sequence conservation was observed with other legume species.
    • Dynamic gene expression patterns and tissue-enriched coexpression clusters were identified.

    Conclusions:

    • The developed red clover transcriptome provides essential genomic resources.
    • Identification of tissue-specific genes and coexpression patterns facilitates future research.
    • This resource supports biotechnological improvements and developmental studies in red clover.