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RAPID COMMUNICATION: Nerve growth factor influences cleavage rate and embryo development in sheep.

M Crispo, P C Dos Santos-Neto, M Vilariño

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    |November 30, 2016
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    Nerve growth factor (NGF) supplementation during sheep oocyte maturation or fertilization improved early embryo development. The optimal NGF concentration was 100 ng/mL, enhancing cleavage and embryo quality.

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    Area of Science:

    • Reproductive Biology
    • Animal Science
    • Cellular and Molecular Biology

    Background:

    • Nerve growth factor (NGF) traditionally regulates neuronal development.
    • Emerging evidence suggests NGF's role in the reproductive system.
    • Understanding NGF's impact on mammalian oocyte and embryo development is crucial.

    Purpose of the Study:

    • To investigate the effects of supplementing Nerve growth factor (NGF) during in vitro oocyte maturation, fertilization, and embryo development in sheep.
    • To determine the optimal concentration and timing of NGF addition for improving reproductive outcomes.
    • To analyze NGF mRNA expression in ovine oocytes and cumulus cells.

    Main Methods:

    • Sheep oocytes underwent in vitro maturation, fertilization, or embryo culture with varying NGF concentrations (0, 100, 1,000 ng/mL).
    • Experiments were conducted across three phases: in vitro maturation (Exp. 1), in vitro fertilization (Exp. 2), and in vitro embryo culture (Exp. 3).
    • NGF mRNA expression was quantified in oocytes and cumulus cells using RT-PCR.

    Main Results:

    • NGF addition during oocyte maturation or fertilization significantly promoted early embryonic cleavage.
    • Supplementation during fertilization notably improved subsequent in vitro embryo development, with 100 ng/mL NGF showing superior effects compared to 1,000 ng/mL.
    • NGF addition during in vitro embryo culture did not yield significant improvements; NGF mRNA was undetectable in oocytes but upregulated in cumulus cells post-fertilization with NGF.

    Conclusions:

    • Nerve growth factor (NGF) supplementation during ovine oocyte maturation and fertilization positively influences in vitro cleavage and embryo development.
    • The findings suggest a potential role for NGF in enhancing oocyte maturation and, more significantly, the fertilization process in sheep.
    • Optimal NGF concentration for observed benefits was 100 ng/mL.