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Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test

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Determining insulin sensitivity from glucose tolerance tests in sheep.

P R Shorten, A B Pleasants, E J Buckels

Journal of Animal Science

|November 30, 2016

Summary

A new mathematical model accurately estimates insulin sensitivity in sheep using a frequently sampled intravenous glucose tolerance test (IVGTT). This method is more cost-effective and simpler than traditional hyperglycemic clamps, aiding metabolic research.

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Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat

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Last Updated: Mar 11, 2026

Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test

Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test

Published on: November 13, 2016

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT

Published on: January 7, 2018

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat

Published on: February 7, 2011

Area of Science:

Metabolic Dynamics
Mathematical Modeling
Animal Physiology

Background:

Insulin sensitivity is crucial for glucose homeostasis.
Accurate measurement of insulin sensitivity is vital for metabolic research.
Current methods like hyperglycemic clamps are resource-intensive.

Purpose of the Study:

To develop and validate a mathematical model for estimating insulin sensitivity using IVGTT in sheep.
To characterize the insulin secretion dynamics during IVGTT in sheep.
To establish IVGTT as a viable, cost-effective alternative to hyperglycemic clamps for insulin sensitivity assessment.

Main Methods:

Developed a mathematical model for insulin and glucose dynamics during IVGTT in sheep.
Fit the model to data from standard and modified IVGTT protocols.
Validated the model's estimations against hyperglycemic clamp (HGC) derived insulin sensitivity.

Main Results:

The IVGTT model accurately characterized sheep insulin secretion dynamics.
IVGTT-derived insulin sensitivity showed significant correlations with HGC-derived values (r=0.43-0.51).
The model successfully detected significant differences in insulin sensitivity during pregnancy and effects of insulin therapy on insulin clearance.

Conclusions:

The developed IVGTT model provides a reliable and more accessible method for determining insulin sensitivity in sheep.
This model can be used to study metabolic changes related to pregnancy and insulin treatment.
IVGTT offers a cost-effective alternative for assessing insulin sensitivity in preclinical research.