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Related Experiment Videos

Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis.

J G Seidman, A Leder, M H Edgell

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1978
    PubMed
    Summary

    Mouse antibody-producing cells contain multiple related but silent variable-region genes alongside the expressed sequence. This suggests the mouse genome may encode a large repertoire of antibody variable genes.

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    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Genomics

    Background:

    • Antibody diversity is crucial for immune response.
    • Mouse kappa light chains, like MOPC-149, are well-studied models for immunoglobulin gene expression.

    Purpose of the Study:

    • To investigate the presence and nature of variable-region gene sequences related to the MOPC-149 kappa light chain in mouse DNA.
    • To determine if antibody-producing cells harbor silent, related gene sequences.

    Main Methods:

    • Identification of EcoRI fragments in mouse DNA.
    • Cloning of specific DNA fragments.
    • Determination of nucleotide sequences of cloned variable-region genes.

    Main Results:

    • At least six EcoRI fragments related to the MOPC-149 kappa light chain variable region were identified.

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  • Two such fragments were cloned and sequenced, revealing variable-region genes from codon 1 to 97.
  • These cloned genes, while closely related, showed sequence differences from each other and the expressed MOPC-149 sequence, particularly in complementarity-determining regions and framework sequences.

    • Conclusions:

    • Antibody-producing cells contain genetic information for their expressed sequence plus several closely related, non-expressed (silent) sequences.
    • The mouse genome may possess extensive sets of variable-region genes for kappa light chains, potentially encoding a significant portion of the antibody repertoire.