Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Species interactions are key to spatiotemporal gene expression and multilayer formation in <i>Stenotrophomonas maltophilia</i> K279a dual species biofilms.

Biofilm·2026

Same author

Engineering and Application of a Thermostable MHETase for PET Depolymerization.

ACS sustainable chemistry & engineering·2026

Same author

Molecular characterisation of extended-Spectrum-Beta-lactamase-producing <i>Klebsiella pneumoniae</i> among children and chicken in the rural Korogwe District, Tanzania.

One health (Amsterdam, Netherlands)·2026

Same author

Role of dienelactone hydrolases in PET biodegradation by flavobacteria <i>Maribacter dokdonensis</i> and <i>Arenibacter palladensis</i>.

Applied and environmental microbiology·2026

Same author

Osmotolerance is a driver of microbial carbon processes in the Elbe estuary.

mSystems·2026

Same author

A novel approach to combat <i>Pseudomonas aeruginosa</i>: repurposing pharmaceuticals for inhibition of phospholipase A.

Microbiology spectrum·2026

Search research articles

Related Experiment Video

Updated: Mar 11, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Screening for Cellulase Encoding Clones in Metagenomic Libraries.

Nele Ilmberger¹, Wolfgang R Streit²

¹Microbiology & Biotechnology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststr. 18, 22609, Hamburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|December 1, 2016

Summary

Identifying novel enzymes is crucial for biotechnology. This study explores cellulolytic enzymes for industrial applications requiring stability under extreme conditions like high temperatures and pH.

Keywords:

Bioethanol Biotechnology Cellulase Ionic liquid Metagenome Renewable energy

More Related Videos

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

Related Experiment Videos

Last Updated: Mar 11, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

Area of Science:

Biotechnology
Enzyme Engineering
Biochemistry

Background:

Modern biotechnology requires novel enzymes capable of functioning under extreme environmental conditions.
Cellulases are vital industrial enzymes used in bioethanol production and textile finishing.
Industrial applications necessitate cellulolytic activity across diverse pH, temperature, and ionic conditions, often utilizing enzyme mixtures.

Purpose of the Study:

To investigate the diversity of cellulolytic enzymes involved in natural cellulose degradation.
To identify novel enzymes with potential for biotechnological applications under harsh conditions.

Main Methods:

Exploration of microbial diversity for cellulase identification.
Characterization of enzyme activity under various stress factors (pH, temperature, solvents).

Main Results:

Discovery of a range of cellulolytic enzymes with varying stability profiles.
Identification of enzymes exhibiting robust activity under non-natural conditions.

Conclusions:

Understanding the natural diversity of cellulases is key to optimizing industrial processes.
Novel cellulases identified show promise for enhancing biotechnological applications requiring enzyme stability.