Methane oxidation in a landfill cover soil reactor: Changing of kinetic parameters and microorganism community...

Changing of CH₄ oxidation potential and biological characteristics with CH₄ concentration was studied in a landfill cover soil reactor (LCSR). The maximum rate of CH₄ oxidation reached 32.40 mol d^-1 m^-2 by providing sufficient O₂ in the LCSR. The kinetic parameters of methane oxidation in landfill cover soil were obtained by fitting substrate diffusion and consumption model based on the concentration profile of CH₄ and O₂. The values of [Formula: see text] (0.93-2.29%) and [Formula: see text] (140-524 nmol kg_soil-DW^-1·s^-1) increased with CH₄ concentration (9.25-20.30%), while the values of [Formula: see text] (312.9-2.6%) and [Formula: see text] (1.3 × 10^-5 to 9.0 × 10^-3 nmol mL^-1 h^-1) were just the opposite. MiSeq pyrosequencing data revealed that Methylobacter (the relative abundance was decreased with height of LCSR) and Methylococcales_unclassified (the relative abundance was increased expect in H 80) became the key players after incubation with increasing CH₄ concentration. These findings provide information for assessing CH₄ oxidation potential and changing of biological characteristics in landfill cover soil.