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Lutibacter litorisediminis sp. nov., isolated from a tidal flat
Sooyeon Park1, Sun Young Yoon1, Min-Ji Ha1
1Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology
|December 1, 2016
Summary
A novel bacterium, Lutibacter litorisediminis sp. nov. OITF-20T, was identified from a South Korean tidal flat. This Gram-negative rod represents a new species within the Lutibacter genus, distinguished by its unique genetic and phenotypic characteristics.
Area of Science:
- Microbiology
- Bacteriology
- Phylogenetics
Background:
- The genus Lutibacter comprises several species of Gram-negative bacteria.
- Understanding bacterial diversity in marine environments is crucial for ecological studies.
- Accurate taxonomic classification relies on detailed phenotypic and genotypic analysis.
Purpose of the Study:
- To characterize a novel bacterial strain, OITF-20T, isolated from a tidal flat.
- To determine the taxonomic position of strain OITF-20T within the genus Lutibacter.
- To propose a new species designation for strain OITF-20T based on distinct characteristics.
Main Methods:
- Isolation and cultivation of strain OITF-20T from a tidal flat environment.
- Phenotypic characterization including optimal growth conditions (temperature, pH, salinity).
- Phylogenetic analysis using 16S rRNA gene sequences.
- Determination of major fatty acids, menaquinone, and polar lipids.
- DNA G+C content determination and DNA-DNA hybridization experiments.
Main Results:
- Strain OITF-20T is a Gram-negative, non-motile, aerobic, rod-shaped bacterium.
- Optimal growth observed at 25°C, pH 7.0-8.0, and 1.0-2.0% NaCl.
- 16S rRNA gene sequence similarity to related Lutibacter species ranged from 97.8% to below 93.4%.
- Distinct fatty acid profile (iso-C15:0 3-OH, C15:1ω6c, iso-C15:0) and predominant menaquinone (MK-6).
- Low DNA-DNA relatedness (17-27%) with type strains of L. crassostreae, L. flavus, and L. litoralis.
- DNA G+C content was 31.1 mol%.
Conclusions:
- Strain OITF-20T exhibits significant phylogenetic and genetic divergence from known Lutibacter species.
- The distinct phenotypic and genotypic properties support the classification of OITF-20T as a novel species.
- The proposed name for the novel species is Lutibacter litorisediminis sp. nov., with OITF-20T as the type strain.
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