Fertilization of Grapevine Based on Gene Expression.

Area of Science:

• Agricultural Science
• Plant Molecular Biology
• Viticulture

Background:

• Gene expression data offers insights into plant growth and metabolic status.
• Accurate monitoring of plant nutritional needs is crucial for effective fertilization strategies.
• Understanding grapevine's specific nutrient requirements at different developmental stages is vital for optimal cultivation.

Purpose of the Study:

• To validate the feasibility of gene information-based fertilization strategies in grapevines.
• To determine the specific nutritional requirements of grapevines during flowering and berry development.
• To assess the response of nutrient uptake and metabolism genes to fertilization.

Main Methods:

• Quantitative polymerase chain reaction (PCR) was used to validate gene expression levels.
• Thirteen nitrogen (N), phosphorus (P), and potassium (K) uptake and metabolism genes were analyzed.
• Gene expression and responses to fertilization were studied during flowering and berry development stages.

Main Results:

• Highly expressed N-P-K uptake and metabolism genes showed positive responses to fertilization.
• Grape quality was significantly improved when fertilization aligned with high gene expression.
• Specific nutrient needs were identified: more P at flowering, N at first berry expansion, less at seed hardening, and P & K at second expansion/veraison.

Conclusions:

• Gene expression-based fertilization is a feasible and workable strategy for grapevines.
• This approach enables precise nutrient management tailored to specific grapevine developmental stages.
• Findings provide novel insights into optimizing vineyard fertilization practices for enhanced grape cultivation.