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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Mar 11, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

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Achieving Plant CRISPR Targeting that Limits Off-Target Effects.

Jeffrey D Wolt, Kan Wang, Dipali Sashital

    The Plant Genome
    |December 1, 2016
    PubMed
    Summary

    CRISPR-Cas9 gene editing in plants shows low off-target mutations. Improving design tools and analyzing plant lines can further enhance specificity for agricultural applications.

    Area of Science:

    • Agricultural Science
    • Molecular Biology
    • Genetics

    Background:

    • CRISPR-Cas9 enables targeted gene modification in plants.
    • Plant genome editing development needs to address potential off-target mutations.
    • Assessing CRISPR-Cas9 specificity is crucial for agricultural applications.

    Purpose of the Study:

    • To evaluate the specificity of CRISPR-Cas9 in plant genome editing.
    • To explore methods for improving CRISPR-Cas9 specificity and reducing off-target effects.
    • To inform regulatory decisions for genome-edited plants in agriculture.

    Main Methods:

    • Analyzing CRISPR-edited plant lines.
    • Developing improved computational tools for predicting off-target mutations.
    • Designing engineered ribonucleoprotein (RNP) complexes.

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    CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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    Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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    Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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    Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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    Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

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    CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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    Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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    Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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    Main Results:

    • CRISPR-Cas9 genome editing in plants demonstrates a low incidence of off-target mutations.
    • Improved computational tools and reagent design show potential for further reducing unintended genetic changes.
    • Enhanced specificity can be achieved by considering genome composition and nuclease fidelity.

    Conclusions:

    • CRISPR-Cas9 is a highly specific tool for plant genome editing.
    • Further refinement of design tools and analysis of plant lines will increase confidence in CRISPR-Cas9 specificity.
    • Validated design tools are essential for regulatory approval of genome-edited crops.