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Microfluidic Chip Fabrication and Method to Detect Influenza
Published on: March 26, 2013
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A novel lab-on-chip platform with integrated solid phase PCR and Supercritical Angle Fluorescence (SAF) microlens
Tran Quang Hung1, Wai Hoe Chin2, Yi Sun3
1Laboratory of Applied Micro and Nanotechnology (LAMINATE) National Food Institute, Technical University of Denmark (DTU-Food), Denmark; Institut Charles Gerhardt UMR 5253 CNRS-UM, Université de Montpellier, Montpellier 34000, France.
Biosensors & Bioelectronics
|December 1, 2016
Summary
This study integrates solid-phase PCR (SP-PCR) with super critical angle fluorescence (SAF) microlens arrays on a lab-on-a-chip (LOC) device. This novel biosensor achieves highly sensitive, on-chip pathogen detection for diverse sample analyses.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Microfluidics
Background:
- Solid-phase PCR (SP-PCR) is crucial for molecular diagnostics but lacks sensitive on-chip detection in lab-on-a-chip (LOC) devices.
- Existing SP-PCR integration into LOC platforms is limited by the absence of portable, sensitive optical detection methods.
- On-line diagnosis capabilities are hindered by current technological limitations in integrated detection systems.
Purpose of the Study:
- To develop a sensitive and portable on-chip optical detection technology for SP-PCR on LOC devices.
- To address the challenge of integrating SP-PCR with advanced detection for on-line molecular diagnostics.
- To enable highly sensitive and multiplexed pathogen detection within a compact LOC platform.
Main Methods:
- Fabrication of miniaturized super critical angle fluorescence (SAF) microlens arrays within a thermoplastic microfluidic chamber.
- Integration of SP-PCR directly onto the fabricated SAF microlens array on the microchip.
- Performance evaluation of the LOC platform for multiplexed SP-PCR assays and sensitivity determination.
Main Results:
- The SP-PCR assay on the LOC platform achieved a high sensitivity of 1.6 copies/µL.
- The SAF microlens array demonstrated high fluorescence collection efficiency, comparable to conventional off-chip laser scanners.
- The integrated system enabled sensitive, multiplexed pathogen detection using low-cost, compact optical components.
Conclusions:
- Combining SP-PCR with SAF microlens arrays on a LOC platform offers a viable solution for sensitive on-chip molecular diagnostics.
- The developed LOC platform provides a high-throughput biosensor for analyzing food, clinical, and environmental samples.
- This technology paves the way for advanced, portable diagnostic tools with broad applicability.
